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Chenodeoxycholic acid pool size determination from children using isotope dilution mass spectrometry
Insights
This study introduces a new, non-radioactive isotope dilution mass spectrometry method to measure chenodeoxycholic acid pool size in children. The validated technique accurately quantifies this important bile acid, benefiting pediatric and prenatal research.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Pediatric Medicine
Background:
- Chenodeoxycholic acid (CDCA) is a primary bile acid crucial for digestion and metabolism.
- Accurate measurement of CDCA pool size is essential for understanding various physiological and pathological conditions, particularly in pediatric populations.
- Existing methods may have limitations, necessitating the development of safer and more precise analytical techniques.
Purpose of the Study:
- To describe and validate a novel isotope dilution mass spectrometry (IDMS) method for determining chenodeoxycholic acid (CDCA) pool size.
- To establish a reliable, non-radioactive assay suitable for clinical use, especially in children and pregnant women.
- To demonstrate the reproducibility of the method across different laboratory settings.
Main Methods:
- Administration of a stable isotopically labeled tracer, (11,12-2H2) chenodeoxycholic acid, to pediatric subjects.
- Measurement of bile enrichment using selected ion monitoring gas chromatography mass spectrometry (GC-MS).
- Validation of the method through independent duplication in two separate laboratories utilizing standard quadrupole mass spectrometers.
Main Results:
- The (11,12-2H2)chenodeoxycholic acid enrichment in patient bile samples ranged from 0.5% to 5%.
- The method demonstrated reproducibility, with successful duplication of results across two independent laboratories.
- The assay proved effective using standard quadrupole mass spectrometers.
Conclusions:
- The developed isotope dilution mass spectrometry method provides a reliable and non-radioactive means to determine chenodeoxycholic acid pool size.
- This technique is particularly advantageous for studies involving pediatric and pregnant populations where radiation exposure is a concern.
- The validated method offers a valuable tool for clinical assessment and research into bile acid metabolism.
Abstract:
An isotope dilution mass spectrometry method is described for determining chenodeoxycholic acid pool size in children. The stable isotopically labeled tracer, (11,12-2H2) chenodeoxycholic acid, was administered orally to children, and the enrichment of bile was measured by selected ion monitoring gas chromatography mass spectrometry. The level of (11,12-2H2)chenodeoxycholic acid enrichment found in the patient samples was in the range of 0.5 to 5%. Data are presented illustrating the duplication of this method in two independent laboratories using standard quadrupole mass spectrometers. This procedure provides the clinician with a non-radioactive method for determining chenodeoxycholic acid pool size which is especially beneficial in studies involving children and pregnant women.