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Related Experiment Videos

'Hydatoxi lualba', an artefact.

A M Polderman, A M van der Knaap, H J Wortel

    European Journal of Obstetrics, Gynecology, and Reproductive Biology
    |June 1, 1984
    PubMed
    Summary

    The Hydatoxi lualba parasite, previously reported in pre-eclampsia, is actually an artefact. These findings demonstrate that cotton swab threads, not a parasite, create the observed

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    Area of Science:

    • Parasitology
    • Hematology
    • Obstetrics

    Background:

    • Previous studies suggested the presence of a parasite, Hydatoxi lualba, in pre-eclamptic patients and their infants.
    • The Hydatoxi lualba parasite was purportedly identified in blood samples using modified staining techniques.

    Purpose of the Study:

    • To investigate the nature of the structures identified as the Hydatoxi lualba parasite in blood samples.
    • To determine the origin of the observed 'parasitic' forms in blood smears from pre-eclamptic pregnancies.

    Main Methods:

    • Blood smears were prepared from control subjects, pre-eclamptic patients, and their infants' cord blood.
    • A modified TBO staining technique was employed on manually cleaned slides.
    • Comparison of findings on manually cleaned versus industrially cleaned slides.

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    Main Results:

    • Structures identified as 'eggs, larva and worms' were demonstrated to be artefacts.
    • These artefacts originated from cotton threads present in cotton swabs used for manual slide cleaning.
    • The artefacts were frequently observed on manually cleaned slides but rarely on industrially cleaned slides.

    Conclusions:

    • The Hydatoxi lualba parasite is not a true biological entity but an artefact of sample preparation.
    • Manual cleaning of slides with cotton swabs can introduce artefacts that mimic parasitic forms.
    • Careful attention to slide preparation techniques is crucial for accurate microscopic diagnosis in hematology and parasitology.