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A microtiter assay for human monocyte activation by lymphokines
Journal of Immunological Methods
|August 3, 1984
Summary
A new assay measures lymphokine activity by tracking increased monocyte adherence to surfaces. This method, using just 20ml of blood, is automatable and suitable for clinical use.
Area of Science:
- Immunology
- Cell Biology
Background:
- Assessing lymphokine activity is crucial for understanding immune responses.
- Existing methods may be complex or require large sample volumes.
Purpose of the Study:
- To develop and validate a novel microtiter assay for quantifying human lymphokine activity.
- To establish a method based on antigen-induced monocyte adherence.
Main Methods:
- Human peripheral blood mononuclear cells were stimulated with antigens (streptokinase/streptodornase or purified protein derivative).
- Lymphokine-induced monocyte adherence to plastic was measured using a 51chromium radioassay.
- The assay was tested with cells from antigen-responsive and unresponsive donors.
Main Results:
- A soluble, non-dialyzable factor increased monocyte adherence.
- This factor was produced by antigen-stimulated cells from responsive donors only.
- The assay demonstrated sensitivity to donor responsiveness.
Conclusions:
- The developed microtiter assay accurately reflects lymphokine activity.
- The assay is sensitive, requires minimal blood volume (20ml), and is suitable for automation and clinical application.
