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Staphylococcal protein A immunoadsorptive column induces mitogenicity in perfused plasma
Journal of Biological Response Modifiers
|January 1, 1984
Summary
Staphylococcal protein A immunoadsorption therapy in cancer patients induced fever and chills. Patient plasma became mitogenic to lymphocytes, suggesting a role in the therapy's immunomodulatory effects.
Area of Science:
- Immunology
- Oncology
- Biotechnology
Background:
- Staphylococcal protein A (SpA) immunoadsorption is a therapeutic approach.
- Its immunomodulatory effects in cancer patients require further investigation.
Purpose of the Study:
- To evaluate the safety and immunomodulatory activity of SpA immunoadsorption therapy.
- To investigate the mechanism behind the observed clinical reactions and immune responses.
Main Methods:
- Phase I clinical trial involving six patients with advanced breast or brain cancer.
- Plasma analysis after perfusion through SpA immunoadsorptive columns.
- Lymphocyte proliferation assays to assess mitogenicity.
Main Results:
- Four out of six patients experienced significant adverse reactions, including fever, chills, and rigors.
- Plasma from these patients exhibited strong mitogenic activity towards normal lymphocytes.
- Mitogenicity correlated with the amount of SpA on the matrix and was not due to leached SpA.
Conclusions:
- SpA immunoadsorption therapy can induce significant clinical and immunological responses in cancer patients.
- A plasma-derived mitogenic component, generated during perfusion, likely contributes to the immunomodulatory activity of SpA therapy.