Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Transport of basic aminoacids in E. coli.

M Di Girolamo, M Busiello, C Cini

    Microbiologica
    |April 1, 1984
    PubMed
    Summary

    E. coli KL16 utilizes two ornithine and one arginine transport systems. Ornithine transporters exhibit poor specificity, while arginine transport is highly specific, with citrulline and arginine inhibiting ornithine uptake.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    HER2-low breast cancer is immune-cold: insights into tumor-infiltrating immune cells and implications for immunotherapy.

    NPJ breast cancer·2025
    Same author

    Spine surgery and complication in familial dysautonomia: a case report.

    Frontiers in surgery·2025
    Same author

    Reliability of core needle biopsy for HER2-low early-stage breast cancer.

    Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·2025
    Same author

    Will smoking on beaches become a thing of the past? Bibione: The first smoke-free beach in Italy.

    Pulmonology·2023
    Same author

    Critical investigation of the pre-surgical anatomic factors that influence the aesthetic result in post-extractive implantology and immediate prosthetic.

    Journal of biological regulators and homeostatic agents·2021
    Same author

    Guided sinus lift: virtual planning of surgical templates for lateral access.

    Journal of biological regulators and homeostatic agents·2021

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Biochemistry

    Background:

    • Understanding amino acid transport in bacteria is crucial for cellular metabolism.
    • Escherichia coli (E. coli) is a model organism for studying bacterial transport systems.
    • Specific transport mechanisms for arginine and ornithine in E. coli KL16 have been previously investigated.

    Purpose of the Study:

    • To characterize the transport systems for ornithine and arginine in E. coli KL16.
    • To determine the specificity of these transport systems.
    • To investigate the inhibitory effects of related amino acids on ornithine transport.

    Main Methods:

    • Utilizing E. coli KL16 as the model organism.
    • Employing transport assays to measure the uptake of ornithine and arginine.
    • Conducting kinetic studies to analyze the specificity and inhibition patterns of the transport systems.

    Main Results:

    • Two distinct transport systems for ornithine were identified in E. coli KL16.
    • One highly specific transport system for arginine was identified.
    • The ornithine transport systems demonstrated poor specificity, being inhibited by citrulline and arginine.
    • Citrulline and arginine exhibited non-competitive inhibition with homotropic cooperativity and synergistic effects on ornithine transport.

    Conclusions:

    • E. coli KL16 possesses multiple, differentially specific transport systems for basic amino acids.
    • The interplay between arginine and ornithine transport highlights complex regulatory mechanisms.
    • These findings contribute to the understanding of nutrient uptake and metabolic regulation in bacteria.

    Related Experiment Videos