Related Experiment Video
Updated: Aug 10, 2026

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells
Published on: February 18, 2014
Diagnostic electron microscopy on reembedded ("popped off") areas of large Spurr epoxy sections
Abstract:
A technique is described in which areas of large Spurr epoxy thick sections are directly popped off of glass slides by inverted Beem capsules filled with polymerized plastic. This technique allows the survey of large regions of tissue with the maintenance of tissue relationships and the selection of specific areas for electron microscopic studies. Within the context of diagnostic electron microscopy well-preserved and representative portions of tissues can be selected as well as individual diagnostic cells or other difficult to find structures. Many small pieces of minced, optimally fixed and processed tissue can also be surveyed by this technique. Thus, one can screen the equivalent of 20 or more standard thick sections in one step. This "pop-off" technique does not appear to alter the ultrastructure of the tissue in any way.
More Related Videos
Related Concept Videos
Fixation and Sectioning
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
Scanning Electron Microscopy
Fundamental Principles
Accelerated...
Preparation of Samples for Electron Microscopy
Immunogold Electron Microscopy
Electron Microscope Tomography and Single-particle Reconstruction
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

