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The decrease of hyaluronate synthesis by anti-inflammatory steroids in vitro.

H Saarni, V K Hopsu-Havu

    The British Journal of Dermatology
    |April 1, 1978
    PubMed
    Summary
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    Anti-inflammatory steroids, including prednisolone and hydrocortisone derivatives, significantly inhibit hyaluronic acid synthesis in human skin fibroblasts. However, these steroids do not impact sulphated glycosaminoglycans synthesis.

    Area of Science:

    • Dermatology
    • Biochemistry
    • Pharmacology

    Background:

    • Anti-inflammatory steroids are widely used in dermatology.
    • Hyaluronic acid and sulphated glycosaminoglycans are key components of the skin extracellular matrix.
    • The specific effects of various anti-inflammatory steroids on skin glycosaminoglycan synthesis are not fully elucidated.

    Purpose of the Study:

    • To investigate the impact of different anti-inflammatory steroids on hyaluronic acid and sulphated glycosaminoglycan synthesis in human skin fibroblasts.
    • To compare the relative potencies of various steroids in inhibiting hyaluronic acid synthesis.

    Main Methods:

    • Human skin fibroblast cultures were treated with a range of anti-inflammatory steroids (prednisolone, hydrocortisone derivatives, dexamethasone, betamethasone) at concentrations from 1 x 10(-10)M to 1 x 10(-6)M.

    Related Experiment Videos

  • Synthesis of hyaluronic acid and sulphated glycosaminoglycans was quantified.
  • Relative inhibitory potencies of steroids on hyaluronate synthesis were calculated.

    • Main Results:

    • All tested steroids reduced hyaluronic acid synthesis by 40-50% of control levels.
    • Steroid concentrations required for inhibition varied, with betamethasone 17-valerate and dexamethasone/betamethasone phosphates being the most potent inhibitors.
    • None of the tested steroids affected sulphated glycosaminoglycan synthesis at the concentrations used.

    Conclusions:

    • Anti-inflammatory steroids potently inhibit hyaluronic acid synthesis in human skin fibroblasts.
    • The observed inhibition of hyaluronic acid synthesis may contribute to the anti-inflammatory effects of these steroids in the skin.
    • Sulphated glycosaminoglycan synthesis remains unaffected by these steroids at the tested concentrations.