Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Immunologic characterization and quantification of haemoglobin A1c.

J Javid, P K Pettis, R J Koenig

    British Journal of Haematology
    |March 1, 1978
    PubMed
    Summary

    A new sheep antiserum specifically targets human glycohaemoglobin (HbA1c), differentiating it from HbA0. This discovery enables a quantitative assay for HbA1c, crucial for diabetes research.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Is There More than One Erythropoietin Receptor? Can the Hematopoietic Effects of EPO Be Dissociated from the Organ-Protective Effects by Carbamylated Erythropoietin?: Derivatives of erythropoietin that are tissue protective but not erythropoietic. Science 305: 239-242, 2004.

    Journal of the American Society of Nephrology : JASN·2023
    Same author

    Effects of hydrogen peroxide, nitric oxide and antioxidants on NF-κB.

    Redox report : communications in free radical research·2016
    Same author

    Simple multiplex RT-PCR for identifying common fusion BCR-ABL transcript types and evaluation of molecular response of the a2b2 and a2b3 transcripts to Imatinib resistance in north Indian chronic myeloid leukemia patients.

    Indian journal of cancer·2016
    Same author

    Clinical significance of TP53 (R72P) and MDM2 (T309G) polymorphisms in breast cancer patients.

    Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·2015
    Same author

    Erratum to: Expression of serum miR-200a, miR-200b and miR-200c as candidate biomarkers in epithelial ovarian cancer and their association with clinicopathological features.

    Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·2015
    Same author

    Expression of serum miR-200a, miR-200b, and miR-200c as candidate biomarkers in epithelial ovarian cancer and their association with clinicopathological features.

    Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·2015

    Area of Science:

    • Immunology
    • Biochemistry
    • Diabetology

    Background:

    • Glycohaemoglobin, specifically HbA1c, is a key biomarker in diabetes management.
    • Distinguishing HbA1c from other haemoglobin fractions is essential for accurate quantification.
    • Existing methods may lack specificity or require complex procedures.

    Purpose of the Study:

    • To develop a specific antiserum against human HbA1c.
    • To evaluate the specificity and utility of this antiserum for HbA1c quantification.
    • To establish a basis for a quantitative assay for HbA1c relevant to diabetes studies.

    Main Methods:

    • Generation of sheep antiserum against purified human HbA1c.
    • Immunological testing of the antiserum against HbA1c, HbA0, HbA1a, HbA1b, and analogous glycohaemoglobins from mouse and dog.

    Related Experiment Videos

  • Assessment of antiserum reactivity following reduction of the sugar ligand's keto group.

    • Main Results:

    • The developed sheep antiserum effectively distinguishes HbA1c from HbA0.
    • Partial cross-reactivity was observed with HbA1a, HbA1b, and non-human glycohaemoglobins.
    • Reactivity was abolished upon reduction of the sugar ligand's keto group, confirming specificity.

    Conclusions:

    • The high immunological specificity of the antiserum provides a reliable method for HbA1c detection.
    • This specificity forms the foundation for a quantitative HbA1c assay.
    • The assay is suitable for both clinical and experimental investigations of diabetes.