The conformation of the polar group of lysophosphatidylcholine in H2O: conformational changes induced by polyvalent...

The conformation of the polar group of egg lysophosphatidylcholine and 1-myristoyl-sn-glycer-3-phosphorylcholine present as micelles in aqueous solution has been studied using NMR methods. In the absence of polyvalent cations the preferred conformation derived from spin-spin coupling constants is similar, but not identical, to that of phosphatidylethanolamine in the crystal structure (cf. Hitchcock, P.B., Mason, R., Thomas, K.M. and Shipley, G.F. (1974) Proc. Natl. Acad. Sci. U.S. 71, 3036--3040). The presence of lanthanides induces a conformational change involving primarily the phosphorylcholine group, e.g. torsion angle alpha5 changes from an all gauche to an approximate trans disposition. The gauche leads to trans transitions observed with torsion angles alpha3 and alpha5 produce a more extended orientation of the polar group (relative to the hydrocarbon chain axis). In the presence of lanthanides the conformation of lysophosphatidylcholine is very similar to that of the diacyl phosphatidylcholines observed in fully hydrated bilayers (cf. Hauser, H., Phillips, M.C., Levine, B.A. and Williams, R.J.P. (1976) Nature 261, 390--394) with the P-N vector at an angle of about 45 degrees to the bilayer.