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Catalytically active enterokinase in human bile.

D A Grant, R W Talbot, J Hermon-Taylor

    Clinica Chimica Acta; International Journal of Clinical Chemistry
    |September 15, 1984
    PubMed
    Summary
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    Active enterokinase, an enzyme resistant to inhibitors, was found in human bile. This suggests a gut-to-bile pathway, potentially triggering acute pancreatitis.

    Area of Science:

    • Biochemistry
    • Gastroenterology
    • Pathophysiology

    Background:

    • Enterokinase is crucial for trypsinogen activation and resists inhibitors.
    • Its presence in bile has not been previously established.

    Purpose of the Study:

    • To detect catalytically active enterokinase in human bile.
    • To elucidate the pathway of enterokinase to bile.
    • To explore the potential role of bile enterokinase in acute pancreatitis.

    Main Methods:

    • Development of a novel one-stage catalytic assay for enterokinase detection.
    • Assay applied to human bile samples from 14 patients.
    • Analysis of potential enterokinase pathways to bile.

    Main Results:

    Related Experiment Videos

    • Catalytically active enterokinase was identified in all 14 human bile samples.
    • Duodeno-biliary reflux could not explain the presence of enterokinase in bile.
    • Findings support a gut-blood-liver-bile enterokinase pathway.

    Conclusions:

    • Active enterokinase is present in human bile.
    • Enterokinase reaches bile via a systemic circulation pathway.
    • Bile enterokinase may initiate necrotising acute pancreatitis upon entering the pancreatic duct.