Carotenoid-deficient maize seedlings fail to accumulate light-harvesting chlorophyll a/b binding protein (LHCP) mRNA

Yellow leaves of chlorophyll-deficient seedlings and white leaves of carotenoid-deficient seedlings contain no detectable light-harvesting chlorophyll a/b binding proteins (LHCP). Chlorophyll-deficient leaves contain plastids which are arrested in development prior to chloroplast formation [Mascia, P.N. and Robertson, D.S. (1978) Planta (Berl.) 143, 207-211] while carotenoid-deficient leaves contain plastids which are arrested in development at a rudimentary stage [Bachmann, M. D., Robertson, D.S., Bowen, C.C., and Anderson, I.C. (1967) J. Ultrastruc. Res. 21, 41-60]. Chlorophyll-deficient leaves have normal levels of nuclear-encoded LHCP mRNA while carotenoid-deficient leaves contain only trace amounts of LHCP mRNA. Similar results were obtained with carotenoid deficiencies caused by nuclear gene mutations and by treatment with the herbicide norflurazon which blocks carotenoid biosynthesis. We conclude that events at early stages of plastid development influence the accumulation of a nuclear-encoded mRNA.