Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Saccharomyces cerevisiae aldolase mutants.

Z Lobo

    Journal of Bacteriology
    |October 1, 1984
    PubMed
    Summary

    Six yeast mutants lacking fructose 1,6-bisphosphate aldolase were identified. These mutants exhibit altered growth on different sugars, indicating aldolase

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A vaccine to prevent herpes zoster and postherpetic neuralgia in older adults.

    The New England journal of medicine·2005
    Same author

    Repression of enzymes of the pentose phosphate pathway by glucose in fission yeast.

    FEBS letters·1999
    Same author

    Std1, a gene involved in glucose transport in Schizosaccharomyces pombe.

    Journal of bacteriology·1998
    Same author

    Suppression of pdc2 regulating pyruvate decarboxylase synthesis in yeast.

    Genetics·1997
    Same author

    Analysis of PFK3--a gene involved in particulate phosphofructokinase synthesis reveals additional functions of TPS2 in Saccharomyces cerevisiae.

    Yeast (Chichester, England)·1994
    Same author

    Expression of the Escherichia coli xylose isomerase gene in Saccharomyces cerevisiae.

    Applied and environmental microbiology·1987

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Yeast Genetics

    Background:

    • Fructose 1,6-bisphosphate aldolase is a key glycolytic enzyme.
    • Understanding its function is crucial for metabolic pathway analysis.

    Purpose of the Study:

    • To isolate and characterize mutants deficient in fructose 1,6-bisphosphate aldolase in Saccharomyces cerevisiae.
    • To investigate the in vivo consequences of aldolase deficiency.

    Main Methods:

    • Isolation of mutants via inositol starvation.
    • Growth assays on various carbon sources (glycerol, alcohol, glucose).
    • Enzyme activity assays and immunological characterization.

    Main Results:

    • Six recessive mutants were identified, all mapping to a single complementation group.
    • Mutants showed reduced aldolase activity (1-5% of wild-type).
    • Accumulation of fructose 1,6-bisphosphate and dihydroxyacetone phosphate was observed, confirming in vivo aldolase absence.

    Conclusions:

    • The isolated mutants are functionally deficient in fructose 1,6-bisphosphate aldolase.
    • This deficiency impacts yeast growth and intermediate metabolite accumulation.
    • The study provides insights into the essential role of aldolase in yeast metabolism.

    Related Experiment Videos