Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[The in vitro system in internal ear research].

M Anniko

    Laryngologie, Rhinologie, Otologie
    |August 1, 1984
    PubMed
    Summary

    Organ culture techniques have advanced inner ear research, enabling detailed studies of embryonic development in mammals. This method allows for controlled investigations into cellular differentiation and tissue interactions crucial for normal morphogenesis.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    In vitro evaluation of the astatinated chimeric monoclonal antibody U36, a potential candidate for treatment of head and neck squamous cell carcinoma.

    European journal of nuclear medicine and molecular imaging·2005
    Same author

    Expression of EGFR, HER2, HER3, and HER4 in metastatic squamous cell carcinomas of the oral cavity and base of tongue.

    International journal of oncology·2005
    Same author

    Hearing loss in relation to round window membrane morphology in experimental chronic otitis media.

    ORL; journal for oto-rhino-laryngology and its related specialties·2001
    Same author

    How can the hooded seal dive to a depth of 1000 m without rupturing its tympanic membrane? A morphological and functional study.

    Acta oto-laryngologica·2001
    Same author

    Localization of nitric oxide synthase isoforms in the human cochlea.

    Acta oto-laryngologica·2001
    Same author

    Detection of nitric oxide in the guinea pig inner ear, using a combination of aldehyde fixative and 4,5-diaminofluorescein diacetate.

    Acta oto-laryngologica·2001

    Area of Science:

    • Developmental biology
    • Otolaryngology
    • Cell biology

    Context:

    • In vitro culture of the embryonic inner ear has a history dating back to the 1920s, initially focusing on avian species.
    • Significant advancements in understanding in vitro conditions for mammalian tissues in the 1960s paved the way for embryonic mammalian inner ear research in the 1970s.
    • Organ culture techniques have been primarily applied to mouse and guinea pig inner ear development, with fewer studies on other species.

    Purpose:

    • To explore the cytodifferentiation and morphogenesis of the embryonic mammalian inner ear anlage using organ culture.
    • To evaluate in vitro techniques for inner ear studies regarding morphology and biochemical parameters.
    • To enable accurate in vitro studies on higher vertebrates, replicating and improving upon in vivo experiments.

    Summary:

    • Organ culture of the mammalian embryonic inner ear allows for detailed examination of neural induction, epithelio-mesenchymal interactions, and neurotrophic interactions.
    • This technique facilitates the study of "fate mapping" and tissue interactions essential for normal inner ear morphogenesis.
    • In vitro studies can investigate vulnerable developmental stages and applied research areas like ototoxicity, focusing on direct tissue effects.

    Impact:

    • Organ culture provides a controlled environment for studying inner ear development, offering insights into cellular and tissue dynamics.
    • The technique allows for precise investigation of factors influencing inner ear morphogenesis and differentiation.
    • While valuable for studying direct tissue effects, organ culture cannot provide information on systemic effects of agents.

    Related Experiment Videos