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Related Experiment Videos

Evaluation of a two-hour method for screening pathogens from stool specimens.

L C Greene, P C Appelbaum, J A Kellogg

    Journal of Clinical Microbiology
    |August 1, 1984
    PubMed
    Summary

    The 2-hour stool-screening test (SST) effectively identifies most bacterial stool pathogens but struggles to exclude non-pathogenic coliforms. Laboratories should consider pathogen prevalence and workflow when adopting this rapid screening method.

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    Area of Science:

    • Clinical microbiology
    • Diagnostic testing
    • Bacteriology

    Background:

    • Accurate identification of bacterial pathogens in stool samples is crucial for patient management.
    • Rapid diagnostic methods are needed to improve laboratory efficiency and patient care.
    • The 2-hour stool-screening test (SST) was developed as a rapid method for pathogen detection.

    Purpose of the Study:

    • To evaluate the diagnostic accuracy of the 2-hour stool-screening test (SST) for identifying potential bacterial pathogens in stool samples.
    • To assess the SST's ability to differentiate between pathogens and non-pathogenic commensal bacteria.
    • To compare the SST's performance with conventional screening methods.

    Main Methods:

    • The SST was used to screen 231 bacterial isolates from stool samples.

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  • Organisms included Salmonella, Shigella, Arizona, Yersinia enterocolitica, Aeromonas hydrophila, Plesiomonas shigelloides, Proteus, Morganella, Providencia, Pseudomonas aeruginosa, and coliforms.
  • Screening results were compared with definitive identification.

    • Main Results:

    • The SST correctly screened all 54 Salmonella isolates.
    • Shigella and Yersinia enterocolitica were often grouped together (SYS) or with other bacteria (SYA).
    • The SST effectively excluded Proteus, Morganella, Providencia, and Pseudomonas aeruginosa but had a high false-positive rate for coliforms, with 69/80 coliforms requiring further testing.

    Conclusions:

    • The SST offers rapid, same-day identification of potential stool pathogens when combined with a quick identification method.
    • Its main limitation is the inability to effectively screen out coliforms, necessitating further testing.
    • Laboratory suitability depends on local pathogen prevalence, workflow, and technologist acceptance.