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H-41, a new minor histocompatibility locus. I. Histogenetic analysis
Journal of Immunology (Baltimore, Md. : 1950)
|December 1, 1984
Summary
The B10.STA12 mouse congenic line possesses a novel minor histocompatibility locus, H-41, distinct from the H-2w13 haplotype. This new locus, H-41a, elicits specific T-cell responses, enabling the differentiation of congenic mouse strains.
Area of Science:
- Immunology
- Genetics
- Transplantation Biology
Background:
- Congenic mouse strains are crucial for dissecting immune responses and genetic loci.
- The B10.STA12 mouse line shares the H-2w13 haplotype with other strains but differs at a minor histocompatibility locus.
- Understanding minor histocompatibility antigens is key to transplantation tolerance and autoimmune disease research.
Purpose of the Study:
- To characterize a newly identified minor histocompatibility locus, designated H-41, in the B10.STA12 mouse congenic line.
- To investigate the immune response, specifically cytolytic T lymphocyte (CTL) activity, directed against the H-41 antigen.
- To determine the genetic basis of skin graft rejection and CTL reactivity between congenic mouse strains.
Main Methods:
- Skin grafting between congenic mouse lines (B10.STA12 and B10.STA10) to assess rejection.
- In vivo immunization and in vitro restimulation to generate and analyze cytolytic T lymphocytes (CTLs).
- Target cell analysis using F1 hybrid mice and various congenic strains to map CTL reactivity.
- Class I major histocompatibility complex (MHC) restriction analysis (Dp restricted).
Main Results:
- B10.STA12 and B10.STA10 mice reject each other's skin grafts, indicating genetic disparity.
- Both strains generate strain-specific CTLs upon immunization, confirming an immune response to foreign antigens.
- CTL reactivity is mapped to the H-41 locus, differentiating B10.STA12 from B10.STA10 and B10.LIB55.
- CTL responses are restricted by Dp, a class I MHC molecule, and show cross-reactivity patterns with other strains.
Conclusions:
- The B10.STA12 congenic line harbors a novel minor histocompatibility locus, H-41, with a distinct allele (H-41a).
- The H-41 locus is responsible for the observed skin graft rejection and CTL responses between B10.STA12 and related strains.
- This finding contributes to the understanding of minor histocompatibility antigen systems and their role in immune recognition.