Related Experiment Video

Updated: Jul 11, 2026

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
19:16

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis

Published on: March 18, 2010

Actinidin hydrolysis of substituted-phenyl hippurates: a quantitative structure-activity relationship and graphics

A Carotti, C Hansch, M M Mueller

    Journal of Medicinal Chemistry
    |November 1, 1984
    PubMed

    Keywords:
    actinidin QSARphenyl hippurate hydrolysisenzyme kineticscysteine protease activity

    More Related Videos

    Development of Inhibitors of Protein-protein Interactions through REPLACE: Application to the Design and Development Non-ATP Competitive CDK Inhibitors10:33

    Development of Inhibitors of Protein-protein Interactions through REPLACE: Application to the Design and Development Non-ATP Competitive CDK Inhibitors

    Published on: October 26, 2015

    Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors09:11

    Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors

    Published on: November 23, 2019

    Related Concept Videos

    Acid Halides to Carboxylic Acids: Hydrolysis01:01

    Acid Halides to Carboxylic Acids: Hydrolysis

    Hydrolysis of acid halides is a nucleophilic acyl substitution reaction in which acid halides react with water to give carboxylic acids. The reaction occurs readily and does not require acid or a base catalyst.
    As shown below, the mechanism involves a nucleophilic attack by water at the carbonyl carbon to form a tetrahedral intermediate. This is followed by the reformation of the carbon–oxygen π bond along with the departure of a halide ion. A final proton transfer step yields carboxylic acid...
    Adrenergic Agonists: Chemistry and Structure-Activity Relationship01:16

    Adrenergic Agonists: Chemistry and Structure-Activity Relationship

    Adrenergic agonists' structure-activity relationship (SAR) determines their selectivity and efficacy. These agonists comprise a phenylethylamine moiety with an aromatic ring and an ethylamine side chain.
    Aromatic ring substitutions: Substituting the aromatic ring with –OH groups at positions 3 and 4 yields catecholamines (e.g., epinephrine), which have a high affinity for adrenoceptors. Hydrogen bonding between –OH groups and receptors enhances adrenergic activity.
    Separation of the aromatic...
    Phase I Reactions: Hydrolytic Reactions01:15

    Phase I Reactions: Hydrolytic Reactions

    Hydrolysis, a cornerstone of phase I biotransformation reactions, uses water to cleave chemical bonds. This process is pivotal in drug metabolism, generating more polar metabolites that can be easily excreted.
    An important hydrolytic reaction is ester hydrolysis. Ester bonds, often found in prodrugs, are broken down, increasing the solubility of drugs like aspirin and lidocaine for more straightforward elimination. Amide hydrolysis is another critical reaction, targeting amide bonds prevalent...