Microbial transformation of testosterone by Aspergillus fumigatus

Microbial transformation has been employed for the preparation of the potentially important 15 beta-hydroxytestosterone which was obtained by fermentation of testosterone with a typical strain of Aspergillus fumigatus. The metabolite was characterized by employing 1H and 13C NMR, mass spectrometry and i.r. analysis. High performance liquid chromatography (HPLC) was used to examine the purity of the product and formation of other minor metabolites.