The ultrastructure and synaptic connections of serotonin-immunoreactive terminals in spinal laminae I and II

In order to study the synaptic relationships of serotonin (5-HT)-containing axons, boutons in laminae I and II of the cat spinal cord were labeled for serotonin with peroxidase-antiperoxidase immunocytochemistry. Labeled boutons were examined with the light microscope and recut into serial ultrathin sections for examination with the electron microscope. Labeled axons exhibiting boutons were sagittally oriented, and were most numerous in lamina I and outer lamina II (IIo) and least numerous in inner lamina II (IIi). Two types of labeled boutons were observed ultrastructurally. A relatively rare, large, scalloped or egg-shaped bouton, which contained many mitochondria and dense core vesicles, was found in laminae I and IIo. A smaller dome-shaped bouton, which contained fewer dense core vesicles and round or pleomorphic, clear vesicles, was found throughout laminae I and II. Both types commonly established symmetrical synaptic contacts with the distal portion of a dendritic tree, rarely with proximal portions or cell somas, and never with axon terminals. The results suggest that there are heterogeneous serotonergic systems that may selectively modify different inputs postsynaptically to functionally different types of neurons in the superficial dorsal horn of the spinal cord.