Separate determination of anticoccidial thiamine analogs by high-performance liquid chromatography

The separate determination of anticoccidial thiamine analogs such as chloroethylthiamine and dimethialium, and thiamine was achieved by reversed-phase high-performance liquid chromatography after conversion to the corresponding thiochromes by alkaline oxidation. Using this method, it was demonstrated that chloroethylthiamine is accumulated in nonproliferating cells of Saccharomyces cerevisiae by the same transport mechanism as for thiamine and dimethialium.