Abnormal fluorescent actin pattern of lazy phagocyte syndromes

Human anti-actin antibodies (from patients with chronic aggressive hepatitis) were used with a second fluorescent anti-serum to study the distribution of actin in granulocytes from ten normal subjects and nine patients with "lazy phagocyte syndromes." Normal cells showed organization of actin at the periphery of the cell within 60 sec of adhering to glass, and this pattern was clearly abnormal in lazy phagocytes. This is a simpler test to confirm abnormal organization of actin in cells from affected patients.