Related Experiment Video
Updated: Aug 16, 2026

Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking
Published on: December 17, 2013
Concentration of a major outer membrane protein at the cell poles in Escherichia coli
Abstract:
Autoradiography of cell envelope ghosts obtained from a strain of Escherichia coli which lacks two major outer membrane proteins has been used to demonstrate the polar concentration of another major outer membrane protein, ompA protein. The beta-lactam antibiotic cephalexin prevents the insertion of newly synthesized ompA protein into the poles but removal of the antibiotic allows the randomly dispersed protein to migrate to the polar and possibly the septal areas of the cell. Labelling of whole cells with bacteriophage K3 has confirmed a polar concentration of ompA protein.
More Related Videos
10:24Separation of the Cell Envelope for Gram-negative Bacteria into Inner and Outer Membrane Fractions with Technical Adjustments for Acinetobacter baumannii
Published on: April 10, 2020
09:12Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System
Published on: March 10, 2020
Related Concept Videos
Cytoskeletal Proteins in Bacteria
Protein Transport to the Outer Chloroplast Membrane
Two models describe the mechanism of precursor recognition and entry across the outer membrane through the TOC complex. Model 1 suggests the newly synthesized precursor binds to the TOC receptor 159 and forms a complex.
Structure of Porins
Formation of Lipopolysaccharides
Bacterial Translocation and Protein Secretion
Chemotaxis in E. coli