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Related Experiment Videos

Endogenous isopropanol: forensic and biochemical implications.

P L Davis, L A Dal Cortivo, J Maturo

    Journal of Analytical Toxicology
    |September 1, 1984
    PubMed
    Summary

    Acetone can be converted into isopropanol through a newly discovered metabolic pathway. This finding is crucial for forensic investigators interpreting alcohol levels in biospecimens.

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    Area of Science:

    • Forensic Toxicology
    • Biochemistry
    • Metabolic Pathways

    Background:

    • Isopropanol and acetone are frequently detected in biospecimens.
    • Unexplained isopropanol findings in individuals without known exposure necessitate origin studies.

    Purpose of the Study:

    • To investigate the origin of isopropanol found in biospecimens.
    • To determine if acetone can be converted to isopropanol in vivo.

    Main Methods:

    • Incubation of acetone, alcohol dehydrogenase (ADH), and NADH at physiological conditions (pH 7.3, 8.8; 37°C).
    • Analysis of reaction products using headspace gas chromatography (GC).
    • Confirmation of product identification via GC/Mass Spectrometry (GC/MS).

    Main Results:

    • Isopropanol is produced from the reduction of acetone, particularly at pH 7.3.
    • This reaction demonstrates an alternative metabolic pathway for acetone.
    • Established reference ranges for endogenous isopropanol in unexposed decedents: blood (1-29 mg/dL), liver (7-59 mg/100 g), brain (2-12 mg/100 g), kidney (6-26 mg/100 g).

    Conclusions:

    • Acetone reduction to isopropanol represents a novel metabolic route.
    • Forensic toxicologists must exercise caution when interpreting low isopropanol concentrations in biospecimens.
    • Consideration of this endogenous production is vital for accurate forensic analysis.

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