Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Urine cannabinoid analysis: an integrated multi-method approach.

D L Black, B A Goldberger, D S Isenschmid

    Journal of Analytical Toxicology
    |September 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A mathematical model of biofilm growth and spread within plant xylem: Case study of Xylella fastidiosa in olive trees.

    Journal of theoretical biology·2024
    Same author

    Screening for diabetes peri-operatively: should we be measuring glucose, HbA<sub>1c</sub> … and insulin?

    Anaesthesia·2022
    Same author

    Traumatic brain injury, omega-3 fatty acids, glutathione and ketosis.

    Anaesthesia·2022
    Same author

    Principles of environmentally-sustainable anaesthesia: a global consensus statement from the World Federation of Societies of Anaesthesiologists.

    Anaesthesia·2021
    Same author

    Mental health, moral injury - and mandatory psychological assessment?

    Anaesthesia·2021
    Same author

    What Can We Do to Increase the Attendance at Our Dental Societies?

    The Dental register·2021

    This study validated a rapid, automated immunoassay for detecting cannabinoids in urine. Positive results were confirmed using high-performance liquid chromatography and thin-layer chromatography, providing reliable forensic evidence.

    Area of Science:

    • Clinical Chemistry
    • Forensic Toxicology
    • Analytical Chemistry

    Background:

    • The Enzyme Multiplied Immunoassay Technique (EMIT) offers rapid, high-volume urine screening.
    • Confirmation methods are crucial for validating immunoassay results in forensic toxicology.

    Purpose of the Study:

    • To adapt the EMIT Urine Cannabinoid Assay for automated analysis.
    • To establish a confirmatory protocol for positive EMIT results using chromatographic techniques.
    • To assess the validity of the combined methods for forensic evidence.

    Main Methods:

    • Automated analysis of the EMIT Urine Cannabinoid Assay on a centrifugal analyzer.
    • Confirmation of 11-nor-delta-9-tetrahydrocannabinol-9-carboxylic acid (THC-COOH) using modified High-Performance Liquid Chromatography (HPLC).

    Related Experiment Videos

  • Further confirmation of THC-COOH presence using High-Efficiency Thin-Layer Chromatography (HETLC).

    • Main Results:

    • The adapted EMIT assay provided cost-effective, rapid, high-volume urine testing.
    • The combined EMIT, HPLC, and HETLC protocol successfully confirmed THC-COOH presence.
    • Positive results across all three methods indicated valid forensic evidence.

    Conclusions:

    • The automated EMIT assay is suitable for high-volume cannabinoid screening.
    • The developed multi-method approach provides reliable confirmation of THC-COOH.
    • This protocol offers valid forensic evidence for cannabinoid use.