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[Morphofunctional characteristics of peritoneal macrophages in mice after cryopreservation]
Abstract:
Peculiarities of changes in morphology of various cells of peritoneal mouse exudate after cryopreservation are described. It is found that in monocytic-macrophage suspension the segment-nuclear neutrophils, reticular and mast cells are the most cryolable, small lymphocytes and monocytes--cryostable. It is demonstrated that after cryotreatment the adhesive activity of the cells and their absorptivity decrease, the pattern of EAC-rosette formation being changed. A method is described of cell restoration through their incubation after cryopreservation for 30 min at 37 degrees C in the medium with serum addition.
Insights
Cryopreservation impacts peritoneal mouse exudate cells, with neutrophils and mast cells being most sensitive. A method involving 30-minute incubation at 37°C with serum can restore cell function after cryopreservation.
Area of Science:
- Immunology
- Cell Biology
- Cryobiology
Background:
- Cryopreservation is essential for preserving biological samples, including immune cells.
- Understanding the cryosurvival of different cell types is crucial for effective sample management.
- Peritoneal exudate cells (PECs) are a heterogeneous population with diverse functions.
Purpose of the Study:
- To investigate the morphological and functional changes in various peritoneal mouse exudate cells following cryopreservation.
- To identify cryolabile and cryostable cell populations within PECs.
- To develop and evaluate a method for restoring cryopreserved PECs.
Main Methods:
- Cryopreservation of peritoneal mouse exudate cells.
- Morphological assessment of cryopreserved cells.
- Evaluation of cell adhesive activity and EAC-rosette formation.
- Incubation of cryopreserved cells in a serum-supplemented medium at 37°C for restoration.
Main Results:
- Segment-nuclear neutrophils, reticular cells, and mast cells exhibited the highest cryolability.
- Small lymphocytes and monocytes demonstrated cryostability.
- Cryopreservation led to decreased cell adhesive activity and absorptivity.
- The pattern of EAC-rosette formation was altered post-cryopreservation.
- A 30-minute incubation at 37°C in a serum-containing medium effectively restored cell function.
Conclusions:
- Peritoneal exudate cell populations vary in their sensitivity to cryopreservation.
- Cryopreservation significantly impairs cellular functions like adhesion and rosette formation.
- A simple post-thaw incubation protocol can successfully restore the viability and function of cryopreserved peritoneal exudate cells.