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A method for the preparation of large intact sheets of intestinal mucosa: application to the study of mouse
A technique for the preparation of entire intestinal mucosal sheets is described that renders the population of crypts accessible for two-dimensional study. We have applied the technique to demonstrate the mosaic crypt populations in the intestinal epithelium of mouse aggregation chimeras, using the lectin Dolichos biflorus agglutinin (DBA) as a strain-specific histochemical marker.
A technique for the preparation of entire intestinal mucosal sheets is described that renders the population of crypts accessible for two-dimensional study. We have applied the technique to demonstrate the mosaic crypt populations in the intestinal epithelium of mouse aggregation chimeras, using the lectin Dolichos biflorus agglutinin (DBA) as a strain-specific histochemical marker.