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Related Experiment Videos

Non-linear curve-fitting programs for Phadezyme IgE PRIST using a Hewlett-Packard HP-97 and a Texas Instruments TI-59

R J Weston, M E Weston, R O Bollinger

    Computers in Biology and Medicine
    |January 1, 1984
    PubMed
    Summary

    A novel curve-fitting program for programmable calculators accurately determines Immunoglobulin E (IgE) levels. This method offers excellent correlation for IgE PRIST results, enhancing diagnostic capabilities.

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    Area of Science:

    • Biochemistry
    • Analytical Chemistry
    • Medical Diagnostics

    Background:

    • Accurate quantification of Immunoglobulin E (IgE) is crucial for diagnosing allergic diseases.
    • Existing methods for IgE determination may require complex calculations or specialized equipment.

    Purpose of the Study:

    • To describe a non-linear curve-fitting program for programmable calculators to determine IgE PRIST results.
    • To evaluate the accuracy and correlation of the developed curve-fitting method.

    Main Methods:

    • Utilized a modified Hoerl's function for non-linear curve fitting.
    • Implemented the program on Hewlett-Packard HP-97 and Texas Instruments TI-59 programmable calculators.
    • Employed the equation ln y = A + B ln x + CxD for curve fitting, with user-defined D value.

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    Main Results:

    • Achieved excellent correlation between reference serum concentrations and curve-fit concentration results.
    • The program successfully calculated curve fit parameters (A, B, C) and an accuracy term (R).

    Conclusions:

    • The described curve-fitting program provides an accurate and accessible method for determining IgE PRIST results.
    • Programmable calculators can be effectively utilized for quantitative immunoassays like IgE determination.