Hepatic and testicular aldehyde dehydrogenase in tumor-bearing mice

The interrelationship between certain dehydrogenases and a hepatic tumor was studied in mice. A rapidly growing hepatoma, Novikoff hepatoma, was transplantable from rats to mice after serial passages in Sprague-Dawley albino mice. Mice inoculated with viable tumor cell suspension were sacrificed 14, 18, 21 or 34 days thereafter. Hepatic cytoplasmic and mitochondrial aldehyde dehydrogenase (ALDH) were measured in addition to liver alcohol dehydrogenase (ADH) and testicular ALDH. Hepatic cytoplasmic and mitochondrial ALDH were markedly inhibited from controls at all time periods studied. Likewise, testicular ALDH was inhibited from respective controls in Novikoff hepatoma-bearing mice. No changes were measurable in hepatic ADH of hepatoma-bearing mice. The enzyme kinetics studied show a reduction in Vmax and an alteration in the apparent Km 34 days after tumor inoculation. Further analyses of hepatic mitochondrial ALDH showed that the inhibition was similarly present in the enzyme with the low and the high Km property. The results suggest that changes in the specific activity and property of ALDH may be a useful tool as a biochemical concomitant to both development and progression of the hepatoma studied.