Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Diffusion and aggregation in biological membranes.

N O Petersen

    Canadian Journal of Biochemistry and Cell Biology = Revue Canadienne De Biochimie Et Biologie Cellulaire
    |November 1, 1984
    PubMed
    Summary

    Membrane component diffusion reveals interactions and aggregation. New fluorescence methods quantify protein aggregation on living cell surfaces, aiding physiological process understanding.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Structural alterations of phospholipid film domain morphology induced by cholesterol.

    Indian journal of biochemistry & biophysics·2012
    Same author

    Effects of second order photobleaching on recovered diffusion parameters from fluorescence photobleaching recovery.

    Biophysical journal·2009
    Same author

    Thermodynamic studies of bovine lung surfactant extract mixing with cholesterol and its palmitate derivative.

    Journal of colloid and interface science·2007
    Same author

    The Sendai virus membrane fusion mechanism studied using image correlation spectroscopy.

    European journal of biochemistry·2001
    Same author

    Sendai virus binds to a dispersed population of NBD-GD1a.

    Bioscience reports·2000
    Same author

    Analysis of membrane protein cluster densities and sizes in situ by image correlation spectroscopy.

    Faraday discussions·2000

    Area of Science:

    • Membrane biophysics
    • Cellular dynamics

    Background:

    • Lateral diffusion of membrane lipids and proteins is crucial for membrane function.
    • Interactions with immobile components constrain diffusion, providing insights into membrane processes.
    • Protein aggregation affects diffusion rates and plays a role in physiological events.

    Purpose of the Study:

    • To discuss fluorescence photobleaching for diffusion measurements.
    • To present a novel fluorescence correlation spectroscopy approach for aggregation analysis.
    • To explore the role of aggregation in membrane dynamics and cellular functions.

    Main Methods:

    • Fluorescence photobleaching measurements.
    • Fluorescence correlation spectroscopy (FCS) variation.
    • Analysis of membrane component aggregation on living cells.

    Main Results:

    • Diffusion measurements characterize surface component interactions.
    • Protein aggregation influences diffusion rates.
    • The presented FCS variation enables quantitative aggregation measurements.

    Conclusions:

    • Direct diffusion measurements are vital for understanding membrane interactions.
    • Quantifying protein aggregation is essential for interpreting diffusion constraints and physiological roles.
    • The novel FCS approach offers a new tool for studying aggregation in living cells.

    Related Experiment Videos