Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

A radioreceptor assay method for insulin.

K F Mori, R J Wood

Journal of Biological Standardization

|October 1, 1984

Summary

A new radioreceptor assay for pharmaceutical insulin products was developed using rat liver membranes. This sensitive and practical method offers reliable potency estimates and stable membrane storage, improving insulin analysis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Spring and latch dynamics can act as control pathways in ultrafast systems.

Bioinspiration & biomimetics·2023

Same author

Consensus exercise identifying priorities for research in the field of general surgery of childhood in the UK.

BJS open·2021

Same author

Lasting lessons learnt in the radiology department from the battle with COVID-19.

Clinical radiology·2020

Same author

Segmental colonic dilation is associated with premature termination of high-amplitude propagating contractions in children with intractable functional constipation.

Neurogastroenterology and motility·2017

Same author

Perching and takeoff of a robotic insect on overhangs using switchable electrostatic adhesion.

Science (New York, N.Y.)·2016

Same author

Titanate nanotubes for reinforcement of a poly(ethylene oxide)/chitosan polymer matrix.

Nanotechnology·2016

Area of Science:

Biochemistry
Pharmacology
Analytical Chemistry

Background:

Accurate quantification of pharmaceutical insulin products is crucial for therapeutic efficacy and patient safety.
Existing bioassays can be time-consuming and resource-intensive.
Development of sensitive and practical in vitro assays is needed.

Purpose of the Study:

To develop and validate a sensitive and practical radioreceptor assay for pharmaceutical insulin products.
To define optimal conditions for assay performance.
To assess the stability of prepared liver membranes for storage.

Main Methods:

Partially purified rat liver plasma membranes were used.
Optimal conditions for the radioreceptor assay were determined.
Intra- and inter-assay variations were calculated.
Correlation with the mouse convulsion bioassay was assessed.
Storage stability of liver membranes at 4°C and -18°C was evaluated.

Main Results:

A sensitive and practical radioreceptor assay method was successfully developed.
Intra-assay variation averaged 7.3% and inter-assay variation averaged 12.2%.
Potency estimates correlated well with the mouse convulsion bioassay.
Liver membranes retained insulin-binding ability after storage for up to 10 weeks at 4°C and 6 months at -18°C.

Conclusions:

The developed radioreceptor assay is a sensitive, practical, and reliable method for quantifying pharmaceutical insulin products.
The assay demonstrates good precision and accuracy, correlating well with established bioassays.
The stability of prepared liver membranes allows for extended storage, enhancing assay practicality.

Related Experiment Videos