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Related Experiment Videos

A radioreceptor assay method for insulin.

K F Mori, R J Wood

    Journal of Biological Standardization
    |October 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    A new radioreceptor assay for pharmaceutical insulin products was developed using rat liver membranes. This sensitive and practical method offers reliable potency estimates and stable membrane storage, improving insulin analysis.

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    Area of Science:

    • Biochemistry
    • Pharmacology
    • Analytical Chemistry

    Background:

    • Accurate quantification of pharmaceutical insulin products is crucial for therapeutic efficacy and patient safety.
    • Existing bioassays can be time-consuming and resource-intensive.
    • Development of sensitive and practical in vitro assays is needed.

    Purpose of the Study:

    • To develop and validate a sensitive and practical radioreceptor assay for pharmaceutical insulin products.
    • To define optimal conditions for assay performance.
    • To assess the stability of prepared liver membranes for storage.

    Main Methods:

    • Partially purified rat liver plasma membranes were used.
    • Optimal conditions for the radioreceptor assay were determined.

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  • Intra- and inter-assay variations were calculated.
  • Correlation with the mouse convulsion bioassay was assessed.
  • Storage stability of liver membranes at 4°C and -18°C was evaluated.

    • Main Results:

    • A sensitive and practical radioreceptor assay method was successfully developed.
    • Intra-assay variation averaged 7.3% and inter-assay variation averaged 12.2%.
    • Potency estimates correlated well with the mouse convulsion bioassay.
    • Liver membranes retained insulin-binding ability after storage for up to 10 weeks at 4°C and 6 months at -18°C.

    Conclusions:

    • The developed radioreceptor assay is a sensitive, practical, and reliable method for quantifying pharmaceutical insulin products.
    • The assay demonstrates good precision and accuracy, correlating well with established bioassays.
    • The stability of prepared liver membranes allows for extended storage, enhancing assay practicality.