Adherence of digested tissue sections for fungal immunofluorescence

Specific immunofluorescence (IF) staining is helpful and often decisive in diagnosis of deep mycoses by examination of host tissue sections. Brightness of fluorescence may be significantly enhanced by prior digestion of tissues with trypsin, but the treatment tends to separate sections from slides. This is avoided by replacement of albumin in the process by an inexpensive commercial product, Elmer's Glue-All. The entire IF staining procedure for fungi in tissue sections is reviewed here, with emphasis on adhesion and digestion of sections.