Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Immobilized glycoconjugates for cell recognition studies.

R L Schnaar

    Analytical Biochemistry
    |November 15, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Myelin-associated glycoprotein modulates apoptosis of motoneurons during early postnatal development via NgR/p75(NTR) receptor-mediated activation of RhoA signaling pathways.

    Cell death & disease·2015
    Same author

    Anti-ganglioside antibody-mediated neuronal cytotoxicity and its protection by intravenous immunoglobulin: implications for immune neuropathies.

    Brain : a journal of neurology·2004
    Same author

    Myelin molecules limiting nervous system plasticity.

    Progress in molecular and subcellular biology·2003
    Same author

    Localization of major gangliosides in the PNS: implications for immune neuropathies.

    Brain : a journal of neurology·2002
    Same author

    Brain gangliosides: functional ligands for myelin stability and the control of nerve regeneration.

    Biochimie·2001
    Same author

    Glycolipids support E-selectin-specific strong cell tethering under flow.

    Biochemical and biophysical research communications·2001

    Cell surface glycoconjugates mediate cell adhesion by binding to carbohydrate receptors. This study models cell recognition using immobilized molecules, demonstrating specific carbohydrate recognition by various cell types.

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Glycobiology

    Background:

    • Cell-cell recognition and adhesion are crucial biological processes.
    • These interactions often involve specific binding between cell surface glycoconjugates and complementary carbohydrate receptors.

    Purpose of the Study:

    • To model and investigate carbohydrate-directed cell adhesion.
    • To demonstrate the ability of different cell types to recognize specific carbohydrates.
    • To explore the role of cell surface carbohydrate receptors in adhesion and cellular responses.

    Main Methods:

    • Immobilizing various glycoconjugates (glycosides, glycoproteins, glycosaminoglycans, glycolipids) onto inert surfaces.
    • Incubating these modified surfaces with intact cells to observe adhesion.

    Related Experiment Videos

  • Utilizing a controlled biochemical approach to study cell-carbohydrate interactions.

    • Main Results:

    • Demonstrated that diverse cell types can recognize and adhere to specific immobilized carbohydrates.
    • Provided evidence for carbohydrate-directed cell adhesion mediated by cell surface receptors.
    • Established a model system for studying specific cell-carbohydrate interactions.

    Conclusions:

    • Specific cell-carbohydrate interactions are mediated by cell surface receptors.
    • This approach offers insights into how cells analyze and respond to their environment.
    • Further research is needed to correlate in vitro findings with in vivo cell-cell recognition.