Microcomputers to control the pH of growing micro-organisms

Area of Science:

• Biochemistry
• Biotechnology
• Control Systems Engineering

Background:

• Negative feedback control is a versatile tool in biochemical processes.
• Microcomputer implementation offers enhanced stability and precision over traditional methods.

Purpose of the Study:

• To demonstrate the utility of microcomputer-based negative feedback control in biochemical applications.
• To present two specific examples showcasing the benefits of this approach.

Main Methods:

• Utilizing microcomputers to generate the transfer function of feedback loops.
• Implementing feedback control for NADH concentration during enzymatic reactions.
• Applying feedback control to maintain constant pH during E. coli cultivation.

Main Results:

• Successfully controlled NADH concentration in an enzymatic reaction.
• Achieved increased yield in E. coli cultivation by maintaining a constant pH.
• Demonstrated the convenience and necessity of computer control for stability and accuracy.

Conclusions:

• Microcomputer-controlled negative feedback systems are highly effective in biochemical applications.
• This technology offers significant advantages in process stability, accuracy, and efficiency.
• The presented examples highlight the practical benefits for scientific research and industrial processes.