Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Interphase nuclear matrix and metaphase scaffolding structures.

C D Lewis, J S Lebkowski, A K Daly

    Journal of Cell Science. Supplement
    |January 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Roadmap to DILI research in Europe. A proposal from COST action ProEuroDILINet.

    Pharmacological research·2023
    Same author

    Pharmacogenetic allele nomenclature: International workgroup recommendations for test result reporting.

    Clinical pharmacology and therapeutics·2015
    Same author

    Direct-to-consumer pharmacogenomic testing assessed in a US-based study.

    The journal of the Royal College of Physicians of Edinburgh·2014
    Same author

    Patients benefit from genetics-guided coumarin anticoagulant therapy.

    Clinical pharmacology and therapeutics·2014
    Same author

    Carriage of the PNPLA3 rs738409 C >G polymorphism confers an increased risk of non-alcoholic fatty liver disease associated hepatocellular carcinoma.

    Journal of hepatology·2014
    Same author

    Is there a need to teach pharmacogenetics?

    Clinical pharmacology and therapeutics·2014

    Nuclear and chromosome protein compositions differ significantly. Nuclei contain more non-histone proteins than chromosomes, with RNA polymerase II associated with the nuclear matrix, suggesting a role in transcription.

    Area of Science:

    • Cell Biology
    • Molecular Biology
    • Biochemistry

    Background:

    • The protein composition of eukaryotic cell structures like nuclei and chromosomes is crucial for their function.
    • Understanding the structural differences and protein associations within these components can elucidate cellular processes.

    Purpose of the Study:

    • To compare the protein compositions of purified metaphase chromosomes and nuclei.
    • To characterize the protein components of nuclear scaffolds and matrices (Type I and Type II).
    • To investigate the association of specific enzymes, such as RNA polymerase II, with these structures.

    Main Methods:

    • Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for protein separation and visualization.
    • Electron microscopy for morphological comparison of nuclear matrices.

    Related Experiment Videos

  • Immunoblotting and enzyme activity assays to detect specific proteins and their functions.

    • Main Results:

    • Nuclei exhibit a more complex protein pattern than chromosomes, with a higher ratio of non-histone proteins to histones.
    • Two specific proteins (X0 and X1) are highly enriched in metaphase chromosomes.
    • The Type I nuclear matrix contains lamina proteins and an internal network stabilized by metalloprotein interactions, while Type II lacks this internal network.
    • RNA polymerase II is tightly bound to the Type I nuclear matrix but not detected in chromosomes or Type II matrix.

    Conclusions:

    • The distinct protein profiles of nuclei and chromosomes reflect their different roles.
    • The nuclear matrix, particularly its internal network, is structurally dynamic and associated with metalloproteins.
    • The binding of RNA polymerase II to the Type I nuclear matrix supports the hypothesis that transcription is closely linked to this structure.