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Renal metabolism in mice of exogenously administered 125I labelled renin
Abstract:
125I labelled mouse submaxillary renin was administered intravenously to anaesthetized male Institute of Cancer Research, USA (ICR) mice in an attempt to determine the organ-related degradation of circulating renin. A specific antirenin antiserum was used for identification. The disappearance rate of labelled renin from plasma was estimated on the basis of a two-compartmental model. The mean half-time of clearance of labelled renin from plasma in intact control and 70% hepatectomized mice was 13.3 +/- 0.8 and 12.0 +/- 0.9 min respectively; these values were not significantly different. The mean half-time of clearance of labelled renin from plasma in nephrectomized mice was 40.0 +/- 2.4 min; this was significantly longer than intact controls and 70% hepatectomized mice. Fifty per cent of the administered renin accumulated in the kidney and 9% in the liver. A small amount of 125I labelled renin was identified in urine, and a large amount of 125I liberated from labelled renin was detected. Since the high performance liquid chromatography profiles showed that plasma renin rapidly moves into the kidney, clearance of circulating renin may take place mainly in this organ.
Insights
The kidney primarily clears circulating renin, as demonstrated by studies using 125I labelled mouse submaxillary renin. Hepatectomy did not affect renin clearance, but nephrectomy significantly prolonged it.
Area of Science:
- Renal Physiology
- Endocrinology
- Biochemistry
Background:
- Circulating renin plays a crucial role in the renin-angiotensin-aldosterone system.
- Understanding renin's organ-related degradation is vital for comprehending its physiological regulation.
Purpose of the Study:
- To investigate the primary organ responsible for the degradation of circulating renin in mice.
- To elucidate the role of the kidney and liver in renin clearance.
Main Methods:
- Intravenous administration of 125I labelled mouse submaxillary renin to anaesthetized ICR mice.
- Estimation of plasma renin disappearance rates using a two-compartmental model.
- Comparison of renin clearance in intact, hepatectomized, and nephrectomized mice.
Main Results:
- Plasma renin clearance half-time was significantly longer in nephrectomized mice (40.0 min) compared to intact (13.3 min) and hepatectomized (12.0 min) mice.
- Fifty percent of administered renin accumulated in the kidney, with 9% in the liver.
- High-performance liquid chromatography indicated rapid movement of plasma renin into the kidney.
Conclusions:
- The kidney is the main organ responsible for the clearance of circulating renin.
- Hepatectomy does not significantly impact renin clearance, suggesting a minimal role for the liver.
- Further research into renal renin handling is warranted.