Application of a backscattered electron image to immunocytochemistry in freeze-cracked tissues

A backscattered electron (BSE) image was applied to the observation of freeze-cracked tissue blocks stained with the indirect immunoperoxidase method. On the cracked surface of the pancreas, the BSE image clearly revealed the immunostained B cell granules as bright spots. A pair of secondary electron and BSE images on a fractured surface are useful for the three-dimensional localization of antigens in cells.