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Updated: Aug 11, 2026

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
Published on: October 24, 2016
[Production of glucose isomerase by Streptomyces phaeochromogenes]
M M Faloci1, O M Yantorno, L A Mazza
1Centro de Investigación y Desarrollo de Fermentaciones Industriales, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Argentina.
Abstract:
The production of glucose isomerase was studied in the Streptomyces phaeochromogenes NRRL B-3559 strain. The influence of the medium composition and the aeration conditions was determined Selection of colonies was necessary to obtain the best results. A grey colony proved to be the most productive one. It was necessary to use the frozen inocula in order to obtain steady reproduction values. It was possible to achieve the maximum enzymatic level (1600 UE/1 min) using a CoCl2. 6H2O concentration of 0.18 g/l. The aeration studies conducted in a mechanically stirred fermentor showed results comparable to those obtained in shaker flasks, operating at 550 rpm and using an air flow of 1 1/1 min. The culture proved to properly aerated since both the cellular oxygen uptake and oxygen demand were similar (Table 1).
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