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A study of DNA melting in concentrated water-alcohol solutions
I A Mikhailenko1, L S Shlyakhtenko
1USSR Cardiology Research Center, Academy of Medical Sciences of USSR, Moscow.
Journal of Biomolecular Structure & Dynamics
|June 1, 1984
Abstract:
The DNA melting profiles with high resolution have been studied for conditions corresponding to the B and A conformations of DNA in water-alcohol solutions. The melting profiles of the A-form and B-form DNA, their mean melting temperatures and melting range width were found to differ. DNA was shown to be heterogeneous in respect of the B-A transition, the GC-rich regions more readily converting into the A form than AT-rich ones. The presence of boundaries between the A and B sections within the transition zone did not smooth off the fine structure of melting profiles.