Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

High-affinity pH-dependent passive Ca binding by myometrial plasma membrane vesicles.

A K Grover, C Y Kwan, E E Daniel

    The American Journal of Physiology
    |January 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Assessing IgG4-related ophthalmic disease and its mimics: a comparison of ACR/EULAR, organ-specific and revised comprehensive diagnostic criteria.

    Journal of clinical pathology·2024
    Same author

    Commentary: The times have changed: Are we listening?

    Indian journal of ophthalmology·2019
    Same author

    Negative velocity fluctuations and non-equilibrium fluctuation relation for a driven high critical current vortex state.

    Scientific reports·2017
    Same author

    Sodium-Calcium Exchanger in Pig Coronary Artery.

    Advances in pharmacology (San Diego, Calif.)·2017
    Same author

    Study of the sign change of exchange bias across the spin reorientation transition in Co(Cr<sub>1-x</sub> Fe <sub>x</sub> )<sub>2</sub>O<sub>4</sub> (x  =  0.00-0.125).

    Journal of physics. Condensed matter : an Institute of Physics journal·2016
    Same author

    Paramagnetic magnetization signals and curious metastable behaviour in field-cooled magnetization of a single crystal of superconductor 2H-NbSe2.

    Journal of physics. Condensed matter : an Institute of Physics journal·2015

    Rat myometrium plasma membranes bind calcium in a pH-dependent way. This high-affinity calcium binding and release may be crucial for smooth muscle excitation-contraction coupling.

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Physiology

    Background:

    • Smooth muscle contraction is regulated by intracellular calcium.
    • Plasma membrane calcium binding sites may influence calcium dynamics.

    Purpose of the Study:

    • To investigate the pH-dependent calcium binding to rat myometrium plasma membranes.
    • To determine the role of these sites in excitation-contraction coupling.

    Main Methods:

    • Isolation of plasma membrane-enriched fractions from rat myometrium.
    • Measurement of calcium binding in response to varying pH and calcium concentrations.
    • Analysis of calcium uptake and release kinetics using A23187.

    Main Results:

    • Rat myometrium plasma membranes exhibit pH-dependent calcium binding, increasing from pH 6.27 to 7.47.

    Related Experiment Videos

  • Calcium binding occurs with high affinity (Kd = 0.28 microM) and a Hill coefficient of 2.37.
  • Rapid calcium uptake and release were observed, suggesting functional significance.

    • Conclusions:

    • The plasma membrane possesses high-affinity, pH-sensitive calcium binding sites.
    • These sites are localized to the plasma membrane, indicated by 5'-nucleotidase activity.
    • The rapid binding and release kinetics suggest a role in smooth muscle excitation-contraction coupling.