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Cell wall-DNA association in Bacillus subtilis.

R J Doyle, A L Koch, P H Carstens

    Journal of Bacteriology
    |March 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

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    Autolysis of Bacillus subtilis cell walls releases DNA, primarily from poles. DNA appears to strongly bind to cell poles, potentially via DNA-membrane complexes, suggesting a role in DNA segregation during cell division.

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Cell Biology

    Background:

    • Cell wall autolysis in Bacillus subtilis releases associated DNA.
    • DNA solubilization is more prominent in later stages of autolysis.
    • Electron microscopy reveals enrichment of cell poles in remaining wall fragments after partial autolysis.

    Purpose of the Study:

    • To investigate the association of DNA with Bacillus subtilis cell walls.
    • To determine the location and mechanism of DNA binding to cell structures.
    • To explore the role of cell poles in DNA segregation during cell division.

    Main Methods:

    • Autolysis of Bacillus subtilis 168 cell walls.
    • Electron microscopy of autolyzed cell wall fragments.
    • Incubation of cell walls with enzymes (subtilisin, DNase, RNase).

    Related Experiment Videos

  • Pulse-chase labeling with N-[3H]acetylglucosamine followed by density gradient centrifugation.

    • Main Results:

    • Partial autolysis enriched for cell poles, with limited DNA solubilization.
    • Enzymatic treatment accelerated DNA release.
    • A proportion of labeled cell material sedimented with DNA-membrane complexes.
    • Radioactivity enrichment in cell poles suggests an affinity for DNA-membrane complexes.

    Conclusions:

    • Cell poles of Bacillus subtilis appear to have a strong affinity for DNA, possibly mediated by DNA-membrane complexes.
    • These findings support the hypothesis that cell poles play a role in DNA segregation during bacterial cell division.