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Related Experiment Videos

A highly sensitive enzyme immunoassay for mouse beta nerve growth factor.

S Furukawa, I Kamo, Y Furukawa

    Journal of Neurochemistry
    |March 1, 1983
    PubMed
    Summary

    A new enzyme immunoassay accurately measures mouse beta nerve growth factor (NGF). This sensitive method detected high NGF levels in the submaxillary gland but not in other tissues or serum.

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    Area of Science:

    • Biochemistry
    • Immunology
    • Neuroscience

    Background:

    • Nerve Growth Factor (NGF) is crucial for neuronal development and survival.
    • Accurate quantification of NGF is essential for understanding its biological roles.
    • Existing bioassays and radioimmunoassays for NGF are often complex and time-consuming.

    Purpose of the Study:

    • To develop a sensitive and rapid enzyme immunoassay for mouse beta nerve growth factor (NGF).
    • To determine the distribution of NGF in various mouse tissues and serum.

    Main Methods:

    • A two-site enzyme immunoassay was developed using anti-mouse beta NGF antibody IgG and an antibody Fab'-linked beta-D-galactosidase conjugate.
    • The assay sandwiches the NGF antigen between the antibody and the enzyme conjugate.

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  • Detection limit of the assay was determined to be as low as 10 pg/ml.

    • Main Results:

    • The developed enzyme immunoassay demonstrated high sensitivity and speed compared to traditional methods.
    • Mouse submaxillary gland exhibited a high concentration of NGF.
    • NGF was not detected in mouse heart, brain, skeletal muscle, or serum.

    Conclusions:

    • The novel enzyme immunoassay provides a reliable and efficient tool for quantifying mouse beta NGF.
    • The findings confirm that NGF is predominantly localized in the mouse submaxillary gland.
    • This study supports previous research indicating the absence of detectable NGF in other mouse organs and serum.