Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Japanese encephalitis virus replication: studies on host cell nuclear involvement.

K R Leary, C D Blair

    Experimental and Molecular Pathology
    |April 1, 1983
    PubMed
    Summary

    Japanese encephalitis virus (JEV) and Venezuelan encephalitis virus (VEV) replication showed distinct sensitivities to actinomycin D, a drug inhibiting RNA synthesis. Virus-specific RNA was localized to the cytoplasm and endoplasmic reticulum in infected cells.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Black Hole Spectroscopy and Tests of General Relativity with GW250114.

    Physical review letters·2026
    Same author

    GW250114: Testing Hawking's Area Law and the Kerr Nature of Black Holes.

    Physical review letters·2025
    Same author

    Improving cosmological reach of a gravitational wave observatory using Deep Loop Shaping.

    Science (New York, N.Y.)·2025
    Same author

    Search for Subsolar-Mass Binaries in the First Half of Advanced LIGO's and Advanced Virgo's Third Observing Run.

    Physical review letters·2022
    Same author

    Point Absorber Limits to Future Gravitational-Wave Detectors.

    Physical review letters·2021
    Same author

    Constraints on Cosmic Strings Using Data from the Third Advanced LIGO-Virgo Observing Run.

    Physical review letters·2021

    Area of Science:

    • Virology
    • Molecular Biology
    • Cell Biology

    Background:

    • Japanese encephalitis virus (JEV) and Venezuelan encephalitis virus (VEV) are significant human pathogens.
    • Understanding viral replication mechanisms is crucial for developing antiviral strategies.
    • The role of host cell machinery, particularly RNA synthesis and localization, in flavivirus and alphavirus replication is not fully elucidated.

    Purpose of the Study:

    • To investigate the impact of actinomycin D (act D) on JEV and VEV replication.
    • To determine the site of virus-specific RNA synthesis and localization within infected cells.
    • To differentiate the replication requirements of JEV and VEV concerning host cell processes.

    Main Methods:

    • Infection of cells with JEV and VEV.

    Related Experiment Videos

  • Treatment with actinomycin D (act D) and mitomycin C at various time points postinfection (PI).
  • Quantification of virus production to assess replication.
  • Autoradiography to detect virus-specific RNA.
  • Cellular fractionation using discontinuous sucrose gradients to localize viral RNA.

    • Main Results:

    • Short pulses of act D did not affect early replication of either JEV or VEV.
    • Continuous act D exposure partially inhibited JEV replication, while VEV replication showed equal sensitivity.
    • JEV replication was unaffected by mitomycin C, indicating DNA synthesis is not required.
    • Virus-specific RNA was exclusively found in the cytoplasm at all PI times.
    • The majority of virus-specific RNA was associated with the endoplasmic reticulum fraction.

    Conclusions:

    • JEV and VEV exhibit differential sensitivity to actinomycin D, suggesting distinct regulatory mechanisms during replication.
    • Viral RNA replication occurs in the cytoplasm and is associated with the endoplasmic reticulum in infected cells.
    • JEV replication is independent of host DNA synthesis.