Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Phorbol myristate acetate-induced macrophage aggregation.

P Badenoch-Jones

    Experimental Cell Biology
    |January 1, 1983
    PubMed
    Summary

    Phorbol myristate acetate (PMA) rapidly aggregates guinea pig macrophages, requiring extracellular Mg2+. This aggregation is inhibited by agents that also affect lymphokine-induced aggregation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Lectin-binding characteristics of related high- and low-metastatic rat mammary adenocarcinoma cell lines.

    Invasion & metastasis·1987
    Same author

    Cellular and secreted tumor plasminogen activator: the effects of NaCl.

    Experientia·1986
    Same author

    Purification and characterization of an inhibitor of plasminogen activator released by rat mammary adenocarcinoma cells.

    European journal of biochemistry·1986
    Same author

    Enhanced plasminogen activator production by highly metastatic variant cell lines of a rat mammary adenocarcinoma.

    Invasion & metastasis·1986
    Same author

    An inhibitor of plasminogen activator produced by tumour cell fusion hybrids.

    The Australian journal of experimental biology and medical science·1985
    Same author

    Characterisation of rat tumour cell hybrids: procoagulant and fibrinolytic activities.

    The Australian journal of experimental biology and medical science·1985

    Area of Science:

    • Immunology
    • Cell Biology
    • Biochemistry

    Background:

    • Phorbol myristate acetate (PMA) is a potent activator of protein kinase C.
    • Macrophage aggregation plays a role in inflammatory and immune responses.
    • Previous studies have investigated PMA-induced cellular responses in various cell types.

    Purpose of the Study:

    • To investigate the mechanism of PMA-induced macrophage aggregation in guinea pigs.
    • To determine the ionic requirements and inhibitory factors involved in PMA-induced macrophage aggregation.

    Main Methods:

    • Guinea pig macrophages were treated with Phorbol myristate acetate (PMA) at nanomole concentrations.
    • The dependence of aggregation on extracellular cations (Mg2+, Ca2+) was assessed.
    • Inhibitory effects of various agents on PMA-induced aggregation were evaluated.

    Main Results:

    • PMA induced rapid aggregation of guinea pig macrophages.
    • Aggregation was dependent on extracellular Mg2+ but not Ca2+.
    • The aggregation was inhibited by agents that also inhibit lymphokine- and ionophore A23187-induced aggregation.
    • The mechanism did not appear to involve arachidonic acid metabolites or respiratory burst products.

    Conclusions:

    • PMA-induced macrophage aggregation is a Mg2+-dependent process.
    • The mechanism shares similarities with PMA-induced neutrophil aggregation.
    • Further research is needed to elucidate the precise molecular pathways involved.

    Related Experiment Videos