Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structural similarities between corresponding heat-shock proteins from different eucaryotic cells.

R Voellmy, P Bromley, H P Kocher

    The Journal of Biological Chemistry
    |March 25, 1983
    PubMed
    Summary

    Heat shock causes cells to produce large heat-shock proteins (hsps) across species. While chicken and fruit fly cells make small hsps, human cells do not, indicating species-specific responses.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Heat Shock Protein Induction in Montastraea faveolata and Aiptasia pallida Exposed to Elevated Temperatures.

    The Biological bulletin·2017
    Same author

    Patency of neck veins following ultrasound-guided percutaneous Hickman line insertion.

    Pediatric surgery international·2013
    Same author

    Ultrasound-guided percutaneous insertion of 2.7 Fr tunnelled Broviac lines in neonates and small infants.

    Pediatric surgery international·2010
    Same author

    Management of end-stage central venous access in children referred for possible small bowel transplantation.

    Journal of pediatric gastroenterology and nutrition·2006
    Same author

    Feedback regulation of the heat shock response.

    Handbook of experimental pharmacology·2006
    Same author

    Anaesthetic outcome and predictive risk factors in children with mediastinal tumours.

    Pediatric blood & cancer·2005

    Area of Science:

    • Cell biology
    • Molecular biology
    • Stress response

    Background:

    • Heat shock triggers cellular defense mechanisms.
    • Cells synthesize heat-shock proteins (hsps) in response to thermal stress.
    • Cross-species comparisons reveal conserved and divergent stress responses.

    Purpose of the Study:

    • To compare the effects of heat treatment on chick embryo fibroblasts, Drosophila embryonic cells, and human lymphoblastoid cells.
    • To investigate the synthesis and structural relationships of heat-shock proteins (hsps) across these species.
    • To analyze changes in RNA synthesis and cell morphology under heat stress.

    Main Methods:

    • Comparative analysis of heat shock protein synthesis in three different cell types.
    • Peptide mapping techniques (tryptic digests, V8 digests) to compare protein structures.

    Related Experiment Videos

  • Measurement of total and poly(A)+ RNA synthesis rates.
  • Microscopic examination of cell morphology.

    • Main Results:

    • All three cell types synthesize large hsps (Mr 70,000 and 84,000-85,000).
    • Chicken and Drosophila cells produce small hsps (Mr 22,000-27,000), but human cells do not.
    • The Mr 84,000-85,000 hsps are structurally related across species, while the Mr 70,000 hsps show less similarity.
    • Heat treatment reduces RNA synthesis rates in all cell types.
    • Chick embryo fibroblasts change shape, but human lymphoblastoid cells do not.

    Conclusions:

    • Species exhibit both conserved and distinct heat shock protein synthesis patterns.
    • Structural similarities exist in large hsps, but differences are noted in smaller hsps and their expression.
    • Heat stress impacts RNA synthesis universally, but morphological changes are species-specific.