Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Immunohistochemistry in cutaneous pathology.

J Thivolet, M Faure

    Journal of Cutaneous Pathology
    |February 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Immunohistochemical (IHC) and immunocytological techniques have expanded beyond initial applications in pemphigus. These methods are now crucial for diagnosing and researching diverse skin diseases, aiding in the study of normal skin components.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Brief report: successive occurrence of T-cell and B-cell lymphomas after renal transplantation in a patient with multiple cutaneous squamous-cell carcinomas.

    The New England journal of medicine·1992
    Same author

    Expression of neuron-specific enolase immunoreactivity by cutaneous and extracutaneous Langerhans-cell histiocytoses ("X").

    The Journal of dermatology·1992
    Same author

    Influence of fixation on human papillomavirus DNA detection in frozen and embedded paraffin lesions by in situ hybridization with biotinylated probes.

    Pathology, research and practice·1992
    Same author

    In vitro HIV-1 entry and replication in Langerhans cells may clarify the HIV-1 genome detection by PCR in epidermis of seropositive patients.

    The Journal of investigative dermatology·1992
    Same author

    Alpha-6 (CD 49f) integrin expression in genetic and acquired bullous skin diseases. A comparison of its distribution with bullous pemphigoid antigen.

    Journal of cutaneous pathology·1992
    Same author

    Anti-CD4 monoclonal antibody therapy in severe psoriasis.

    Journal of autoimmunity·1992

    Area of Science:

    • Dermatology
    • Immunohistochemistry
    • Immunocytology

    Background:

    • Immunologic findings in skin diseases have significantly expanded since the initial detection of pemphigus antibodies in 1964.
    • Dermatology extensively utilizes immunohistochemical (IHC) and immunocytological techniques for both clinical diagnosis and scientific research.
    • Techniques have evolved from indirect immunofluorescence (IF) to include immunoenzymatic methods.

    Purpose of the Study:

    • To highlight the expanding role and utility of immunohistological techniques in dermatology.
    • To emphasize the value of IHC and immunocytology in diagnosing and researching skin conditions.
    • To underscore the potential of these methods in studying normal skin constituents using specific antibodies.

    Main Methods:

    Related Experiment Videos

  • Application of immunohistological techniques on skin biopsies.
  • Utilizing immunocytological techniques.
  • Employing serological methods in conjunction with tissue analysis.
  • Development and use of specific polyclonal and monoclonal antibodies for identifying skin antigens, collagen, and keratin.

    • Main Results:

    • Immunohistological techniques have become indispensable tools in dermatological research and diagnosis.
    • While not all findings are disease-specific, IHC provides valuable insights when correlated with serological data.
    • The study of normal skin constituents (e.g., antigens, collagen, keratin) is a growing field enhanced by these antibody-based methods.

    Conclusions:

    • Immunohistochemistry and related techniques are vital for modern dermatological practice.
    • These immunological methods offer significant advantages for understanding skin diseases and normal skin structure.
    • Continued advancements in antibody technology promise to further increase the importance of IHC in dermatology.