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Determination of relative antigen-antibody avidities by radial immunodiffusion
Radial immunodiffusion can be used to determine relative antigen-antibody avidities in exactly the same way as demonstrated previously for quantitative immunoelectrophoresis (Birkmeyer et al., 1981). Antigen-antibody interactions of greater avidity result in a greater value of (delta Area/delta [Antigen]) in plots of immunoprecipitin circle area versus antigen concentration while interactions of equal avidity will yield equal values of (delta Area/delta [Antigen]). This was demonstrated using antigens of different weight ranging from 8000 to 66,000.
Radial immunodiffusion can be used to determine relative antigen-antibody avidities in exactly the same way as demonstrated previously for quantitative immunoelectrophoresis (Birkmeyer et al., 1981). Antigen-antibody interactions of greater avidity result in a greater value of (delta Area/delta [Antigen]) in plots of immunoprecipitin circle area versus antigen concentration while interactions of equal avidity will yield equal values of (delta Area/delta [Antigen]). This was demonstrated using antigens of different weight ranging from 8000 to 66,000.