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Endotoxin fails to induce procoagulant in C3H/HeJ exudate macrophages
Abstract:
Monocytes and macrophages from a variety of animal species produce procoagulants upon stimulation with endotoxin in vitro. While C3H/HeJ mice and their cells have exhibited refractoriness to known effects of phenol-water extracted endotoxin, two recent reports indicate that the cells of these mice produce procoagulants in a normal manner in response to endotoxin. This study compares the responsiveness of cells of C3H/HeJ and C3H/HeN mice to phenol-water extracted endotoxin and to disrupted gram-negative cell walls. Phenol-water extracted endotoxin had no mitogenic effect on spleen cells and failed to elicit procoagulant synthesis in exudate macrophages from C3H/HeJ mice. In contrast it was an effective stimulant for spleen cells and exudate macrophages of C3H/HeN mice. Gram-negative cell walls were an effective stimulant for spleen cells and macrophages from both mouse strains. I conclude that exudate macrophages of C3H/HeJ mice do not respond to endotoxin by producing procoagulant.
Insights
C3H/HeJ mice macrophages do not produce procoagulants when stimulated by endotoxin, unlike their C3H/HeN counterparts. This refractoriness highlights specific immune response differences in endotoxin-mediated pathways.
Area of Science:
- Immunology
- Cell Biology
- Microbiology
Background:
- Monocytes and macrophages synthesize procoagulants when stimulated by endotoxin.
- C3H/HeJ mice typically show refractoriness to endotoxin's effects.
- Recent studies suggest C3H/HeJ cells may respond normally to endotoxin.
Purpose of the Study:
- To compare the responsiveness of C3H/HeJ and C3H/HeN mouse cells to endotoxin and bacterial cell walls.
- To investigate endotoxin-induced procoagulant synthesis in macrophages from different mouse strains.
Main Methods:
- Stimulation of mouse spleen cells and exudate macrophages with phenol-water extracted endotoxin.
- Stimulation with disrupted gram-negative cell walls.
- Comparison of procoagulant synthesis and mitogenic effects between C3H/HeJ and C3H/HeN mice.
Main Results:
- Phenol-water extracted endotoxin failed to stimulate procoagulant synthesis in C3H/HeJ macrophages and had no mitogenic effect on their spleen cells.
- The same endotoxin effectively stimulated C3H/HeN macrophages and spleen cells.
- Disrupted gram-negative cell walls stimulated both C3H/HeJ and C3H/HeN cells, indicating responsiveness to other bacterial components.
Conclusions:
- Exudate macrophages from C3H/HeJ mice are refractory to endotoxin-induced procoagulant production.
- C3H/HeJ mice exhibit specific unresponsiveness to phenol-water extracted endotoxin, not a general defect in responding to bacterial stimuli.
