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Related Experiment Videos

Cellular Li+ opens paracellular path in toad skin: amiloride blockable effect.

J Aboulafia, S M Sanioto, F Lacaz-Vieira

    The Journal of Membrane Biology
    |January 1, 1983
    PubMed
    Summary
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    Lithium increases toad skin permeability to sodium, potassium, and sucrose by affecting intracellular calcium levels. This suggests lithium disrupts cellular structures, impacting barrier function.

    Area of Science:

    • Physiology
    • Membrane Transport
    • Cell Biology

    Background:

    • The short-circuit current model is used to study epithelial transport.
    • Lithium's effects on biological membranes are not fully understood.
    • Paracellular pathways play a crucial role in epithelial barrier function.

    Purpose of the Study:

    • To investigate the effect of lithium (Li) on the permeability of toad skin.
    • To elucidate the mechanism underlying Li-induced permeability changes.
    • To explore the role of intracellular calcium and cytoskeleton in Li's action.

    Main Methods:

    • Short-circuit current measurements on isolated toad skin.
    • Flux measurements of 24Na, 42K, and 14C sucrose.
    • Pharmacological blockade of Li entry using amiloride.

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    Main Results:

    • Lithium significantly increased the efflux of Na, K, and sucrose across toad skin.
    • The effect of Li on sucrose permeability suggests an increase in paracellular pathway permeability.
    • Amiloride blocked the Li-induced increase in permeability, indicating intracellular mediation.
    • A potential mechanism involves Li-induced disruption of cellular calcium balance and cytoskeleton.

    Conclusions:

    • Lithium enhances non-specific permeability in toad skin via intracellular mechanisms.
    • Changes in intracellular calcium and cytoskeleton organization are implicated in Li's effect.
    • This study provides insights into the molecular basis of lithium's impact on epithelial barriers.