Solid-phase immunoassays using chimera antibodies prepared with monoclonal or polyclonal anti-enzyme and anti-erythrocyte antibodies

The possibility of using covalently prepared chimera antibodies in solid phase immunoassays was tested. The antibody specific for the antigen to be quantified was conjugated with a polyclonal or monoclonal antibody specific for an enzyme or for erythrocytes. Using these conjugates, labelling was done by enzyme/anti-enzyme or erythrocyte/anti-erythrocyte interactions. The chimera antibodies were used for quantitation of human IgE and for titration of human anti-Echinococcus granulosus antibody by solid-phase immunoassays, and were found to possess equal or higher amplification than conventionally prepared enzyme-antibody conjugates. A washing device for erythroadsorption test is also described. It allows such a test to be expressed in quantitative terms, as is the case in enzyme-immunoassays.