In vitro, non enzymatic labelling of histone H1 with [14C] acetyl CoA

Histones H 1A and H 1B, in nuclear homogenates or after purification, are able to be acetylated or labelled by [14C] acetyl CoA in the absence of enzyme, as shown after blotting into nitrocellulose sheets. The reaction is pH-dependent, resistant to 2 M NaCl and does not occur at high ionic strength.